Part:BBa_K1469001:Experience
Part is user sequenced: no illegal restriction sites and no mutations.
Protein expression was checked by SDS-PAGE and Western Blot.[http://2014.igem.org/Team:Saarland/4_step#WesternBlot]
Localisation of a GFP tagged Has2 protein variant was checked by fluorescence microscopy.[http://2014.igem.org/Team:Saarland/4_step#Fluorescence]
No catalytic activity probably due to misfolding and intracellular accumulation.[http://2014.igem.org/Team:Saarland/4_step#Rheometer]
Applications of BBa_K1469001
BioBrick is designed for production of high molecular mass hyaluronic acid (HMM-HA) in a Bacillus megaterium based expression system. HMM-HA is supposed to have inhibitory effects on cancer cell lines.
User Reviews
UNIQe5462573dfd68fe1-partinfo-00000000-QINU UNIQe5462573dfd68fe1-partinfo-00000001-QINU