Coding

Part:BBa_K1469001:Experience

Designed by: Philip Hartz   Group: iGEM14_Saarland   (2014-10-02)


Part is user sequenced: no illegal restriction sites and no mutations.

Protein expression was checked by SDS-PAGE and Western Blot.[http://2014.igem.org/Team:Saarland/4_step#WesternBlot]

Localisation of a GFP tagged Has2 protein variant was checked by fluorescence microscopy.[http://2014.igem.org/Team:Saarland/4_step#Fluorescence]

No catalytic activity probably due to misfolding and intracellular accumulation.[http://2014.igem.org/Team:Saarland/4_step#Rheometer]

Applications of BBa_K1469001

BioBrick is designed for production of high molecular mass hyaluronic acid (HMM-HA) in a Bacillus megaterium based expression system. HMM-HA is supposed to have inhibitory effects on cancer cell lines.

User Reviews

UNIQb59ec0889f8f9142-partinfo-00000000-QINU UNIQb59ec0889f8f9142-partinfo-00000001-QINU